The main focus of the department is to provide high quality service and improve standards.
The efficiency of the laboratory in meticulously following internationally recommended techniques, adhering to various elaborate inbuilt control protocols and guidelines reflects in the accurate test results.
All samples, particularly CSF and body fluids, are investigated in detail. This has helped us to detect rare diseases such as cryptococcal meningitis, tubercular brain abscess, disseminated nocardiosis, cardiac hydatidosis and systemic histoplasmosis.
State-of-the-art techniques :
NASBA (Nucleic Acid Sequence Based Amplification) assays started for molecular diagnosis of CMV and HIV (quantitative).
Geneprobe for molecular diagnosis of TB (RNA amplification assay) BacTAleart-3D also includes automotion for rapid culture of mycobacteria. Rapid accuprobe assay done for species identification of mycobacteria.
Automation in blood culture (BacT/Alert), the first in north India .
VITEK-2 has been acquired by upgrading Vitek-1, for automation in bacterial identification and antimicrobial sensitivity (with MIC values).
Direct fluorescence assay (for M. tuberculosis)
Profiles for viral hepatitis (A, B, C and E). Ours is probably the only laboratory in the city that confirms hepatitis B virus surface antigen positive cases with a confirmatory neutralisation assay.
Rapid diagnostic tests for HIV (all HIV positives are confirmed with 3 different ELISAs as per WHO recommendations), cryptococcal meningitis and leptospirosis.
ELISA for dengue fever, mycobacterial infections, amoebiasis, etc.
Assay for minimum inhibitory concentration (MIC) using the Etest, to obtain accurate knowledge of antibiotic sensitivity
Schlichter's Test: The efficacy of antibiotics against bacterial isolates in vivo is ensured by the Schlichter's test. This is important in patients with bacterial endocarditis, those facing antibiotic therapy failure and in determining the efficacy of switching from parenteral to oral therapy before discharge.
Quality Control protocols followed, as per international standards.
Antibiotic sensitivity is done as per the Clinical and Laboratory Standards Institute (CLSI) with American Type Culture Collection (ATCC) controls.
Enzyme Immuno Assay (EIA) controls evaluated in every run.
Computerised data records and archives.
A standard operating procedures (SOP) manual was published as a reference document for all technical staff to follow. This was upgraded as per NABL standards.
Molecular Diagnosis of Infectious Diseases
The nucleic acid sequence based assays (NASBA) are being done for the following: -
HIV (Qualitative and Quantitative)
TB - generpobe assay
The earliest detection of the reactivation of CMV or Tuberculosis shall be known by detecting its messenger RNA in almost any sample. This assay is better than PCR by not amplifying the nucleic acid sequences from dead organisms and has minimal threat of carryover contamination.
ELISA work station
Anaerobic work station